Product Overview :
HRV 3C Protease Activity assay kit utilizes the ability of a 3C Protease (derived from a HRV rhinovirus-14, EC: 3.4.22.28) to cleave a chromogenic peptide substrate to release a chromophore (pNA) which can be easily quantified using a microplate reader. This assay kit is simple, rapid and can detect HRV 3C Protease activity as low as 50 ng in samples and of purified proteins.
Description :
Human rhinovirus (HRV) infections are the most frequent causative agents of common cold and various other upper respiratory tract infections. Rhinoviruses are members of the picornavirus family, which have a positive-sense, single-stranded RNA genome that is translated into a single polyprotein precursor. In the case of HRVs, the viral polyprotein is mainly processed by the proteases (2A and 3C) to generate functional proteins and enzymes.
Storage :
Store the kit at -80°C, protected from light. Briefly centrifuge small vials at low speed prior to opening. Read the entire protocol before performing the assay.
HRV 3C Protease Assay Buffer: Store at 4°C. Bring to room temperature before use.
HRV 3C Protease (Positive Control): Aliquot and store at -80°C for long term. Avoid repeated freeze/thaw cycles.
Kit Components :
HRV 3C Protease Assay Buffer, 25 ml
HRV 3C Protease (10 μg, Positive Control), 10 μl
HRV 3C Protease Substrate, 500 μl
pNA Standard (0.1 M), 20 μl
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