| Cat# | Product Name | Price |
|---|---|---|
| Kit-0419 | HDAC8 fluorometric drug discovery Kit | Inquiry |
| Kit-0433 | HDAC8 Activity Assay Kit | Inquiry |
| Kit-0434 | HDAC8 Assay Kit | Inquiry |
| Kit-1744 | HDAC8 Fluorogenic Assay Kit | Inquiry |
| Kit-1961 | HDAC8 Inhibitor Screening Assay Kit | Inquiry |
| Kit-2253 | HDAC8 Activity Fluorometric Assay Kit | Inquiry |
HDAC8 is a histone deacetylase and a member of the histone deacetylase family. It can remove acetyl tags from the N-terminal, transform histones into non acetylated forms, and regulator gene transcription and chromatin structure.
The biological activity of HDAC8 plays a role in many cellular processes, including cell proliferation, apoptosis, differentiation, cell cycle, and gene expression. It is a key factor in the growth and transformation of cancer cells, participating in the occurrence and development of various tumors such as the breast, lung, colon, stomach, ovary, and prostate. The overexpression of HDAC8 is associated with tumor growth, invasion, and metastasis.
The main methods for measuring HDAC8 activity are fluorescence detection and radiometry. The fluorescence detection method uses fluorescent substrates to measure HDAC8 activity, where the deacetylation of the fluorescent substrate can release fluorescent dyes, while the acetylation state converted to a non-fluorescent substrate cannot release fluorescence. The activity of HDAC8 can be determined by measuring the fluorescence intensity released.
The radioassay method determines the activity of HDAC8 by using radiolabeled substrates. When the substrate is deacetylated, radioactive isotopes are released, and measuring the emitted radiation energy can obtain the value of HDAC8 activity.
In addition, there are various HDAC8 activity detection methods such as mass spectrometry, Proteolytic Release assays, silicon electrode assay, enzyme-linked immunosorbent assay (ELISA), and immunoblotting. Among them, mass spectrometry is an emerging technology widely adopted in recent years, which can provide rich information and better understand the substrate specificity and characteristic regions of enzymes.
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