Product Overview :
Histone deacetylases (HDACs) play a central role in controlling cell cycle regulation, cell differentiation, and tissue development. HDAC1 is ubiquitously expressed and plays a role in cellular senescence, cardiomyocyte differentiation and hypertrophy, aging of the liver, myelination, and adult neurogenesis to name a few functions. The HDAC1 IP & Activity Assay Kit provides an antibody-based method to specifically immunoprecipitate the HDAC1 complex from cells & tissues and to measure HDAC1 activity fluorometrically. HDAC1 is immunoprecipitated from cell, nuclear or tissue extract(s) using HDAC1 antibody followed by capturing the complex on protein-A/G beads. The immunoprecipitated complex is incubated with the HDAC substrate. Only the deacetylated substrate is cleaved by the Developer to produce a fluorophore, which can be easily analyzed using a fluorescence plate reader.
Applications :
Immunoprecipitation of HDAC1 complex from cell, nuclear, or tissueextract(s)
Measurement of HDAC1 activity of immunoprecipitated complex/purified enzyme
Screening for activators or inhibitors of HDAC1
Kit Components :
HDAC Assay Buffer; Cell Lysis Buffer; HDAC Substrate; Developer; AFC (7-amino-4-trifluoromethyl coumarin) Standard (1 mM); Rabbit HDAC2 Antibody (0.2 mg/ml); Rabbit IgG (Control Antibody) (0.5 mg/ml); Protein A/G Sepharose Beads; Positive Control (Jurkat Cell Lysate) (125 μg)
Detection method :
Fluorescence (Ex/Em=380/500 nm)
Features & Benefits :
Simple, fast and convenient.
Only kit on the market for both IP as well as measuring activity of HDAC1
PDF