| Cat# | Product Name | Price |
|---|---|---|
| Kit-0983 | Fluorimetric HDAC Activity Assay Kit (Green) | Inquiry |
| Kit-0412 | HDAC/SIRT Chemiluminescent drug discovery Kit | Inquiry |
| Kit-0413 | HDAC colorimetric activity assay Kit | Inquiry |
| Kit-0414 | HDAC fluorometric activity assay Kit | Inquiry |
| Kit-0415 | HDAC fluorometric cellular activity assay Kit | Inquiry |
| Kit-0420 | Green HDAC fluorometric activity assay Kit | Inquiry |
| Kit-0422 | HDAC Activity Assay Kit | Inquiry |
| Kit-0423 | HDAC Activity Fluorometric Assay Kit | Inquiry |
| Kit-0424 | HDAC Activity/Inhibition Colorimetric Assay Kit | Inquiry |
| Kit-0425 | HDAC Activity/Inhibition Fluorometric Assay Kit | Inquiry |
HDAC (Histone Deacetylase) is an important class of enzyme proteins that primarily deacetylate histones and participate in biological processes such as gene transcription regulation and epigenetic regulation.
HDAC is a type of active enzyme that catalyzes the N-terminal deacetylation of histone proteins, leading to biological processes such as gene silencing and gene expression regulation. HDAC is composed of multiple subtypes, including Classes I, II, III, and IV. Among them, Class I and II HDAC contain Zn2 domains that specifically deacetylate extended N-terminal histone proteins, while Class III HDAC can rely on NAD cleavage acetylation. These different types of enzyme proteins each have different biological functions.
1. Gene transcription regulation
HDAC enzyme proteins make histones more tightly wrap DNA by removing the acetylation modification of histone protein N-terminal, which limits the binding of RNA polymerase and gene startup. The increase in HDAC gene expression often leads to the silencing of certain genes, which are often key target genes for tumor cell growth and transformation.
2. Cell cycle regulation
HDAC enzymes can also have an impact on other cell cycle regulatory proteins, for example, they can disable modifications such as phosphorylation, acylation, and membranization of cell cycle regulatory proteins, affecting cell cycle and proliferation.
3. Others
HDAC enzymes are also involved in other biological processes, such as cell apoptosis and differentiation.
1. Sequence dependent protease method
The sequence dependent protease method, also known as the color heat method, is currently a commonly used method for measuring HDAC activity. Methods based on the continuous hydrolysis of HDAC on the artificial substrate, the substrate was dipeptide Boc Lys (Ac) - AMC. Under the action of enzyme, the deacetylated Boc Lys AMC was separated; and at a certain temperature, AMC generates fluorescence signals due to the generation of fluorescence; detect the fluorescence value through a flow or plate reader to determine the HDAC enzyme activity in the experiment.
2. Fluorescence polarization method
Fluorescence polarization method is a new type of HDAC activity measurement method, which differs from sequence dependent protease method in that the substrate in this method is the dipeptide Boc Lys (Ac) -7-AMC, which is an improved version of the previous substrate and can improve the signal-to-noise ratio. Based on the molecular structure of the substrate, fluorescence polarization method can distinguish between acetylated and non deacetylated substrates. By changing the fluorescence polarization degree, the HDAC enzyme activity of the sample can be monitored and measured.
3. Charge transfer method
Another method for measuring HDAC enzyme activity is the charge transfer method, which is based on changes in the N-terminal acylation state of the target protein, using N '- diisopropyl lysine dipeptide as a semi synthetic substrate. The acylated lysine forms a transfer complex with the amino acid connected to one side, and covalent bond transfer occurs. By using this method to detect changes in the acylation state of target proteins, the HDAC enzyme activity in routine test samples can be determined.
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