| Cat# | Product Name | Price |
|---|---|---|
| Kit-0407 | HAT Activity Colorimetric Assay Kit | Inquiry |
| Kit-0408 | HAT Activity Fluorometric Assay Kit | Inquiry |
| Kit-0409 | HAT Inhibitor Screening Assay Kit | Inquiry |
| Kit-0410 | Histone Acetyltransferase Activity Colorimetric Assay Kit | Inquiry |
| Kit-0411 | Histone Acetyltransferase Inhibitor Screening Assay Kit | Inquiry |
| Kit-2262 | HAT (H4) Activity Fluorometric Assay Kit | Inquiry |
Histone Acetyltransfer (HAT) is a protein that regulates the expression of regulator gene. HAT transfers acyl groups to histones without acylation. This process is considered to be one of the key steps of transcriptional activation. In the field of cell biology, the detection of HAT activity is very important.
Histone Acetyltransfer (HAT) is a protein enzyme that adds acetyl molecules to the N-terminus of histone proteins. Histone is a core protein that, together with DNA, forms chromosomes. The N-terminal of histones can regulator gene expression by acetylation. HAT enzyme can increase histone acetylation, thereby promoting the expression of certain genes. Therefore, research on HAT enzymes helps us understand the regulation of gene expression.
The biological effects of HAT enzyme are reflected in the transcriptional activation of genes. HAT enzyme can acetylate the amino terminus of histone proteins, thereby altering gene expression levels. The biological effects of HAT enzyme are tissue specific, and the biological activity of HAT enzyme varies in different organs and tissues of the human body. By detecting the activity of HAT enzyme, researchers can understand the expression level of HAT in specific tissues and provide assistance for the diagnosis and treatment of clinical diseases.
The commonly used method for measuring the activity of Histone Acetyltransferase is to use luminescent substrate or UV absorption method, among which luminescent substrate method is one of the most commonly used methods.
Luminescent substrate method: Luminescent substrate method is a commonly used method for measuring HAT enzyme activity. This method relies on enzyme catalyzed acetylation transfer to produce quantifiable products. This method typically uses isolated enzyme reaction systems (including matrix histones, enzymes, and acetylated substrates) and quantifiable luminescent substrates to determine the products of acetylation reactions. Among them, the substrate is usually a molecule containing a luminescent group, which generates a fluorescence signal when activated by an activator or enzyme catalysis. The higher the substrate concentration, the stronger the signal. The advantages of luminescent substrate method are high sensitivity and convenience, but it is not as accurate as UV absorption method.
UV absorption method: UV absorption method is one of the traditional techniques for measuring HAT enzyme activity. This method measures the UV absorption of acetylhistone products generated by HAT catalyzed acetylation transfer to determine enzyme activity. The main advantage of this method is its high accuracy, but it is not as fast as the luminescent substrate method.
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