| Cat# | Product Name | Price |
|---|---|---|
| Kit-0403 | GZMB Activity Fluorometric Assay Kit | Inquiry |
| Kit-0404 | GZMB Assay/Inhibitor Screening Fluorometric Kit | Inquiry |
GZMB, also known as Granzyme B, is an important enzyme protein. It is mainly produced within human immune cells, such as NK cells and T lymphocytes. It can directly attack viruses, tumors, and infected cells, and is also involved in various biological processes such as cell regulation, damage repair, and other immune responses. This article will introduce the biological activity characteristics of GZMB, as well as the methods and principles for measuring GZMB activity.
1. Biological activity of GZMB
GZMB has multiple biological activity characteristics. The most important thing is that it can participate in cell apoptosis and signal transduction. In the immune response of the human body, when infected or tumor cells enter the body, immune cells will release GZMB protein into the infected or tumor cells, disrupting cell function from within and causing cell death. In addition, GZMB also has certain functions in producing and regulating tumors, inflammatory reactions, and autoimmune diseases.
2. GZMB activity determination method
In clinical trials, the GZMB activity measurement method can help diagnose some diseases and monitor treatment effectiveness. The most widely used GZMB enzyme activity detection kit is currently labeled with fluorescent groups. Among them, Ac-IEPD-AMC was used as the substrate and treated with buffer solution to add it to the test sample for reaction. Fluorescein acetate ester (AMC) can be generated with the enzyme activity of GZMB, and a fluorescence reaction can be generated. By measuring with a fluorescence meter, the GZMB enzyme activity can be calculated.
The experimental steps for detecting GZMB activity are as follows:
1). Extract cells or plasma: Extract GZMB using cell eluent or plasma with sodium acetate buffer.
2). Preparation of reactants: The GZMB activity assay kit contains GZMB substrates and buffer solution, which are prepared and added to the plate well.
3). Add sample: Add the extracted sample to the plate well and react with the GZMB substrate.
4). Fluorescence detection: Add fluorescein acetate ester and calculate the GZMB enzyme activity.
3. The detection principle of GZMB
The GZMB enzyme activity detection method is based on the principle of fluorescein acetate acylation reaction and is an effective enzyme-linked immunosorbent assay (ELISA) technique. The substrate Ac-IEPD-AMC, as a non-fluorescent substrate in its initial state, undergoes a strong fluorescence reaction after enzymatic hydrolysis to generate AMC under the action of GZMB. Under the stimulation of a fluorescence detection system, the fluorescence signal can quantitatively detect the activity of GZMB enzyme.
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