Product Overview :
The GTPase Assay kit is a homogeneous, bioluminescent assay that is used to measure the activities of GTPases and their associated proteins (GEFs and GAPs) in vitro. The assay quantitates the amount of GTP remaining after a GTPase reaction.
With the GTPase Assay kit, you can measure intrinsic GTPase activity, GAP-stimulated GTPase activity, GAP activity and GEF activity. The kit contains optimized reaction buffers, GTPase/GAP Buffer and GEF buffer, for performing GTPase and GAP reactions, and GEF reactions, respectively.
Kit Components :
rGTP, 10mM 50µl
DTT, 100mM 100µl
GTPase/GAP Buffer 5ml
GEF Buffer 5ml
GTPase Buffer 5ml
GTPase Reagent, 500X 15µl
Detection Reagent 10ml
ADP, 10mM 0.5ml