| Cat# | Product Name | Price |
|---|---|---|
| Kit-0397 | Fluoro GST Activity Kit | Inquiry |
| Kit-0398 | Glutathione S-Transferase Assay Kit | Inquiry |
| Kit-0399 | GST Activity Colorimetric Assay Kit | Inquiry |
| Kit-0400 | GST Activity Fluorometric Assay Kit | Inquiry |
GST is the abbreviation for glutathione S-transferase. It is an important enzyme widely present in organisms. GST is involved in many physiological processes in the human body, including regulating cell growth, apoptosis, and detoxification. Today we will delve deeper into the biological activity of GST, as well as the methods and principles for measuring GST activity.
GST is mainly involved in the process of regulating metabolism within cells. It can transfer cellular toxins to the glutathione metabolites generated by glutathione (GSH), helping the body eliminate harmful chemicals. At the same time, GST can also reduce oxidative stress in the brain and reduce neuronal damage by transferring glutathione to the metabolites of neurons.
In addition, GST can also digest the pressure caused by exogenous compounds. The expression of GST in cells can protect cells by regulating protein translation and transcription processes, which makes GST play a crucial role in the metabolism and detoxification of chemicals, pollutants, and other substances that can cause oxidative damage and their metabolites, thereby maintaining cell integrity and stability.
Due to the important role of GST, the development of GST activity measurement methods and detection principles is of practical significance, which can help us explore the specific functions of GST in organisms. The following is an introduction to commonly used methods and principles for measuring GST activity.
2.1 Determination of GST and Glutathione Reaction
The GST glutathione reaction determination method is currently the most commonly used method for determining GST activity, which detects the influence of GST on the formation rate of products catalyzed by glutathione methylation. The operation steps are as follows:
Add an appropriate mixture of GST sample and GSH to the buffer solution;
Add aromatic group sink (Z -) and observe the change or display amplitude of the absorption peak at 340nm wavelength;
Use an automatic wavelength meter to record the absorbance value every 10 seconds;
By comparing the changes in absorbance values before and after the reaction with the control under the same conditions, the activity of GST can be calculated.
2.2 Fluorescence Resonance Energy Transfer (FRET) detection method
The Fluorescence Resonance Energy Transfer (FRET) detection method is a highly sensitive and accurate GST activity measurement method used to determine the fluorescent substances released from the matrix by specific genes or proteins with GST activity. Therefore, this method has wide applications in fields such as biochip analysis and drug screening. The specific operating steps are as follows:
Add a fluorescent substrate to the reaction system containing GST;
Two fluorescent substances will undergo resonance energy transfer, forming a fluorescence signal;
Only when GST hydrolyzes the substrate, the fluorescent substrate is released, and the two fluorescent substances separate. The fluorescence intensity emitted is proportional to the activity of GST;
By observing the fluorescence intensity released, the activity of GST can be determined.
We are here to answer any question you may have