| Cat# | Product Name | Price |
|---|---|---|
| Kit-0381 | Glutamic Oxaloacetic Transaminase Activity Assay Kit | Inquiry |
| Kit-0382 | Glutamic Oxaloacetic Transaminase Activity Fluorometric Assay Kit | Inquiry |
Glutamic Oxalacetic Transaminase (GOT) is an enzyme widely present in the liver, myocardium, and muscles. GOT is a very important enzyme in humans, animals, and plants. Its main function is to assist in amino acid metabolism, converting glutamic acid into α- Ketoglutaric acid. Although its role in the human body is important, when it leaves the normal range, it can cause irreversible damage to the human body, so detecting its level is extremely important.
The biological activity of GOT is the result of various enzyme catalyzed reactions. It is a key enzyme for the synthesis and breakdown of amino acids, essential for protein synthesis and fibrinogen activation reactions. Its main function is to catalyze the transfer of amino acids into the pyruvate ring α- The conversion of ketoglutaric acid and the formation of pyruvate in the pyruvate ring ensure human health. Under normal circumstances, the level of GOT in human cells is relatively stable, not too much or too little. However, in the progression of certain diseases, the level of GOT may rapidly increase, thereby proving the progression of the disease.
The methods for measuring GOT activity include direct and indirect methods, where the direct method is based on observing the optical changes of the enzyme reaction, while the indirect method is based on measuring the increase in GOT generated by certain substrate reactions. The direct determination method mainly utilizes the reaction between GOT and glutamic acid oxalamide, which produces glutamic acid and glutamic acid under the catalysis of GOT α- Ketone polyamide, accompanied by NADH consumption, continuously reacts with azo pairs to form a purple red compound, and the GOT concentration is calculated by measuring its absorbance. The indirect method is mainly based on the analysis of myocardial fibrinolytic enzyme, transaminase substrate, coagulation factor and other indicators, as a reference index to evaluate liver function and detect some liver diseases.
The detection principle is mainly based on the fact that GOT is an enzyme present in the liver, myocardium, and muscles. When liver, heart, and muscle tissues are damaged, GOT activity continues to increase, and these GOTs are released into the bloodstream, which may ultimately lead to serious negative consequences. Therefore, the level of GOT in the blood can usually be used as an indicator for evaluating liver, heart, and muscle damage.
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