Product Overview :
GDH Activity Assay Kit is used for measuring glucose dehydrogenase activity.
Description :
Glucose 1-dehydrogenase (NAD+) (EC 1.1.1.118) is an enzyme that catalyzes the chemical reaction: D-glucose + NAD+ ↔ D-glucono-1,5-lactone + NADH + H+. This enzyme belongs to the family of oxidoreductases, specifically those acting on the CH-OH group of donor with NAD+ or NADP+ as acceptor. GDH Activity Assay Kit provides a convenient tool for sensitive detection of the GDH in a variety of samples. The GDH present in sample will recognize D-glucose as a specific substrate leading to a proportional color development. The activity of GDH can be easily quantified colorimetrically (λ = 450 nm). This assay detects GDH activity as low as 0.01 mU with our unit definition.
Usage :
For research use only (RUO)
Storage :
Store the kit at -20°C, protect from light. Allow Assay Buffer to warm to room temperature before use. Briefly centrifuge vials prior to opening. Read the entire protocol before performing the assay.
Kit Components :
GDH Assay Buffer. Cap code: WM. 25 mLGlucose (2 M). Cap code: blue. 1 mLDeveloper (lyophilized). Cap code: red. 1 vialGDH Positive Control (lyophilized). Cap code: green. 1 vialNADH Standard (0.5 μmol, Lyophilized). Cap code: yellow. 1 vial
Detection method :
Colorimetric
Compatible Sample Types :
Biological Fluid, Cell Culture Supernatant, Culture Medium, Fermentation Medium, Food Sample, Plasma, Serum, Tissue Culture Supernatant, Urine
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