Product Overview :
Glucokinase mediates phosphorylation of glucose. Allosteric agents which could activate GK activities or increase GK affinity to glucose will be a useful lead molecular to develop novel therapeutics for anti-diabetes. Glucokinase kit is a convenient, high-throughput method for enzyme-luminescence detection of glucokinase activity, which can be used for screening of glucokinase modulators.
Description :
Glucokinase (GK, hexokinae IV, D) mediates phosphorylation of glucose at the sixth carbon position in the initiation step of glycolysis and glycogen synthesis in the liver. GK plays central role in blood glucose homeostasis and has low affinity to glucose in physiological conditions. The increased glucose level induces the conformational change of GK from inactive form to active form to keep glucose homeostasis. Allosteric agents which could activate GK activities or increase GK affinity to glucose will be a useful lead molecules to develop novel therapeutics for anti-diabetes. Recently, several GK activating agents were reported as a candidates for anti-diabetic drugs. This user-friendly and highly sensitive glucokinase assay kit is a useful tools to explorer novel glucokinase modulators for anti-diabetes drug development. This luminescent system has several advantages such as non-radioactive and homogeneous, no requirement of specific antibodies, short hand-on times, cost-effective HTS assay system over other assay systems.
Kinase reaction: Substrate (glucose) + ATP --> Phospho- substrate + ADP
Signal detection: Luciferin + ATP +1/2 O2 --> Oxyluciferin + AMP +CO2+PPi
Storage :
All components may be stored at –80°C. If unable to do so, store liquid Glucokinase (Component A) and Control GK activator (Component C) at –80°C and all other components at –20°C until use. Properly stored products will be active for at least 6-12 months.
Kit Components :
Comp A. Glucokinase (Liver type)
Comp B. Assay buffer
Comp C. Control GK activator
Comp D. Detection reagent
Comp E. Deionized water
White microplate
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