Product Overview :
FBP activity assay kit provides a quick, reliable test that allows the measurement of FBP enzymatic activity in various samples. In this Assay, FBP hydrolyzes Fructose-1,6-Bisphosphate into Fructose-6-Phosphate (F6P). F6P is used in an enzymecoupled reaction which reduces a chromophore forming a product with a stable signal that can measured at OD = 450 nm. The assay is simple, sensitive and can detect lower than 100 µU of FBP in variety of samples.
Description :
Fructose-1,6-Bisphosphatase (FBP) (EC 3.1.3.11) is a rate-limiting enzyme for gluconeogenesis. It hydrolyzes Fructose-1,6-Bisphosphate releasing inorganic phosphate and Fructose-6-Phosphate that is further used in the synthesis of glucose. In humans, Fructose-1,6- Bisphosphatase can be found in the form of two isozymes: FBP1 and FBP2. FBP1 is mainly expressed in liver and kidneys, while FBP2 is expressed in all tissues. Deficiency of Fructose-1,6-Bisphoshatase causes hypoglycemia, metabolic acidosis, and unexpected death in infants. Recent studies have found elevated FBP levels subjects dealing with obesity and diabetes. Furthermore, inhibition of FBP increases glucose utilization and insulin sensitivity. Therefore, FBP could serve as a potential target for treating type II diabetes (insulin resistance).
Applications :
• Measurement of Fructose-1,6-Bisphosphatase Activity in various tissues/cells.
• Analysis of gluconeogenesis pathway.
• Mechanistic studies of Molecular and/or Cell biology processes (diabetes, gluconeogenesis)
Storage :
Store kit at -20°C, protected from light. Warm all buffers to room temperature before use. Briefly centrifuge all small vials prior to opening.
Kit Components :
FBP Assay Buffer 25 ml
FBP Substrate 1 vial
FBP Converter 1 vial
FBP Developer 1 vial
FBP Probe 1 vial
FBP Converter 1 vial
FBP Substrate 1 vial
FBP Developer 1 vial
Compatible Sample Types :
• Animal tissues: Liver, Kidney etc.
• Cell culture: HeLa, Jurkat, etc.
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