Product Overview :
Free Glycerol Assay Kit uses a single Working Reagent that combines glycerol kinase, glycerol phosphate oxidase and color reactions in one step. The color intensity of the reaction product at 570nm or fluorescence intensity at λem/ex = 585/530nm is directly proportional to glycerol concentration in the sample.
Description :
GLYCEROL [GLYCERIN or GLYCERINE, C3H5(OH)3] is widely used in foods, beverages and pharmaceutical formulations. It is also a main byproduct of biodiesel production. Simple, direct and automation-ready procedures for measuring glycerol concentrations find wide applications.
Applications :
Direct Assays: glycerol in biological samples (e.g. serum and plasma).
Drug Discovery/Pharmacology: effects of drugs on glycerol metabolism.
Food and Beverages: glycerol in food, beverages, pharmaceutical formulations etc.
Usage :
For research use only (RUO)
Storage :
The kit is shipped on ice. Store all components at -20°C. Shelf life of 12 months after receipt.
Kit Components :
Assay Buffer: 24 mL
Enzyme Mix: 500 µL
ATP: 250 µL
Dye Reagent: 220 µL
Standard: 100 µL 100 mM Glycerol
Detection method :
Colorimetric, Fluorometric
Features & Benefits :
Sensitive and accurate. Use as little as 10 µL samples. Linear detection range in 96-well plate: 10 to 1000 µM (92 µg/dL to 9.2 mg/dL) glycerol for colorimetric assays and 2 to 50 µM for fluorimetric assays.
Simple and convenient. The procedure involves addition of a single working reagent and incubation for 20 min at room temperature, compatible for HTS assays.
Improved reagent stability. The optimized formulation has greatly enhanced the reagent and signal stability.
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