Product Overview :
FFA is both a product of fat hydrolysis and a substrate for fat synthesis. The concentration of FFA in serum is related to lipid metabolism, glucose metabolism and endocrine function. FFA combines with copper ions to form fatty acid copper salts, which are dissolved in chloroform; the content of free fatty acids can be calculated by measuring the content of copper ions by the copper reagent method.
Kit Components :
Extraction solution: 50 mL×1 bottle, store at 2-8°C
Reagent 1: 50 mL × 1 bottle (self-provided), stored at room temperature, one day before the experiment, take a glass bottle and prepare it according to the ratio of n-heptane: anhydrous methanol: chloroform = 24:1:25 (self-provided), cover Mix immediately.
Reagent 2: 16 mL×1 bottle, stored at room temperature
Reagent 3: powder × 2 bottles, store at 2-8°C, add 32 mL of absolute ethanol to each bottle before use to fully dissolve, and store at 2-8°C for one week.
Standard product: powder × 1, stored at room temperature, 10mg palmitic acid. Before use, transfer the reagent to a 10 mL glass bottle and add 7.8 mL of chloroform to dissolve it fully, which is a 5 μmol/mL palmitic acid standard solution.
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