Product Overview :
Formic acid (HCOOH) is the simplest carboxylic acid and occurs naturally, notably in ant and bee venom. It is normally present at low levels in blood and urine (up to ~ 0.5 mM) but can be present in concentrations as high as 5 mM in acute methanol poisoning. Environmental exposure to formaldehyde can also elevate the blood and urine levels of formate. In the Formate Assay Kit, formate is oxidized to generate a product resulting in color formation (λ = 450 nm) proportional to formate concentration. The kit provides a convenient means for detecting formate in biological samples such as in serum or plasma, cells, culture and fermentation media. There is no need for pretrea;ent or purification of samples.
Applications :
The assay can detect formate in the range from 0.5 to 10 nmols/well.
Kit Components :
Formate Assay Buffer; Formate Enzyme Mix; Formate Substrate Mix; Formate Standard (100 mM)
Target Species :
Mammalian
Detection method :
Absorbance (450 nm)
Features & Benefits :
Simple procedure; takes ~60 minutes;
Fast and convenient;
Kit contains all necessary reagents for accurate measurement of formate levels in a number of sample types
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