Product Overview :
Activation of caspases plays a central role in apoptosis. The CaspGLOW;Fluorescein Active Caspase-8 Staining Kit provides a convenient means for sensitive detection of activated caspase-8 in; Living cells. The assay utilizes the caspase-8 inhibitor, IETD-FMK, conjugated to FITC (FITC-IETD-FMK) as a fluorescent in situ marker. FITC-IETD-FMK is cell permeable, nontoxic, and irreversibly binds to activated caspase-8 in apoptotic cells.
Applications :
Sensitive detection of activated caspase-8 in living cells.
Kit Components :
FITC-ATAD-FMK; Wash Buffer; Z-VAD-FMK
Target Species :
Mammalian
Detection method :
Fluorescence microscopy, Flow cytometry and fluorescence plate reader (Ex. = 485 nm and Em. = 535 nm)
Features & Benefits :
Simple one-step procedure; takes only 1-2 hours; Fast and convenient; The FITC label allows for direct detection of the activated caspases in apoptotic cells
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