Product Overview :
EZH2 is a histone methyltransferase that exhibits methylation activity toward H3-K27.
Description :
The EZH2 Homogeneous Assay Kit is designed to measure activity of the EZH2 for screening and profiling applications, using AlphaLISA technology. The key to the EZH2 Homogeneous Assay Kit is a highly specific antibody that recognizes methylated substrate. With this kit, only three simple steps on a microtiter plate are required for methyltransferase detection. First, a sample containing EZH2 enzyme is incubated with the histone substrate for one hour. Next, acceptor beads and primary antibody are added, then donor beads, followed by reading the Alpha-counts.
Applications :
Great for studying enzyme kinetics and screening small molecular inhibitors for drug discovery and HTS applications.
Storage :
12 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.
Kit Components :
EZH2: 100 µg; -80°C100 µM S-adenosylmethionine: 400 µl; -80°C Primary antibody 8*: 5 µl; 4°C Histone mixture (his-tagged): 400 µl; -80°C 4x HMT assay buffer 2: 3 ml; -20°C 4x Detection buffer 2: 2 ml; -20°C
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