Product Overview :
EZH2 is a histone methyltransferase that exhibits methylation activity toward H3-K27.
Description :
The EZH2 Direct Activity Chemiluminescent Assay Kit is designed to measure activity of the EZH2 complex (EZH2/EED/SUZ12/RbAp48/AEBP) for screening and profiling applications. The key to the EZH2 Direct Activity Assay Kit is a highly specific antibody that recognizes methylated Histone H3K27. With this kit, only three simple steps on a microtiter plate are required for methyltransferase detection. First, S-adenosylmethionine is incubated with a sample containing assay buffer and methyltransferase enzyme for one hour. Next, primary antibody is added. Finally, the plate is treated with an HRP-labeled secondary antibody followed by addition of the HRP substrate to produce chemiluminescence that can then be measured using a chemiluminescence reader.
Applications :
Great for studying enzyme kinetics and screening small molecular inhibitors for drug discovery and HTS applications.
Storage :
12 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.
Kit Components :
EZH2/EED/SUZ12/RbAp48/AEBP2: 25 µg; -80°C400 µM S-adenosylmethionine: 250 µl; -80°C Primary Antibody 6: 12.5 µl; -80°C Secondary HRP-labeled antibody 2: 10 µl; -80°C 4x HMT assay buffer 2: 3 ml; -20°C Blocking buffer: 50 ml; +4°C HRP chemiluminescent substrate A (2 components): 6 ml each; +4°C 96-well plate precoated with histone substrate: 1 plate; +4°C
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