Description :
Enteropeptidase (Enterokinase, EC 3.4.21.9) is a serine protease involved in activation of trypsinogen to trypsin, which in turn results in the activation of various digestive enzymes. It recognizes a highly specific amino acid sequence ‘DDDDK’ and cleaves after the lysine residue. High specific activity of Enteropeptidase has been utilized in cleaving a variety of native or fusion protein tags containing the above recognition motif. In Enteropeptidase Activity Assay Kit, we have utilized a peptide substrate containing the Enteropeptidase recognition sequence along with a fluorescent label ‘AFC’. Enteropeptidase catalyzes the cleavage of this substrate and releases the AFC molecule, which can be easily quantified by measuring its fluorescence at Ex/Em = 380/500 nm. This assay kit is simple and rapid and can detect Enteropeptidase activity as low as 1 mU.
Applications :
Measurement of Enteropeptidase activity in biological samples or purified Enteropeptidase activity.
Removing tag from recombinant proteins having recognition motif.
Kit Components :
Enteropeptidase Assay Buffer; Enteropeptidase Substrate (10 mM, in DMSO); Human Enteropeptidase (Positive Control); AFC Standard (1 mM)
Detection method :
Fluorescence(Ex/Em = 380/500 nm)
Features & Benefits :
Simple & convenient procedure.
Can detect enteropeptidase activity as low as 1 mU.
Kit contains all necessary reagents for measuring enteropeptidase activity.
PDF