| Cat# | Product Name | Price |
|---|---|---|
| Kit-0312 | Dipeptidyl peptidase IV Inhibitor Screening Assay Kit | Inquiry |
| Kit-0313 | DPP IV Immunocapture Activity Assay Kit | Inquiry |
| Kit-0314 | DPP4 Activity Fluorometric Assay Kit | Inquiry |
| Kit-0315 | DPP4 Inhibitor Screening Kit | Inquiry |
| Kit-0316 | DPPIV drug discovery Kit | Inquiry |
| Kit-0317 | DPPIV/CD26 Assay Kit | Inquiry |
DPP4 refers to dipeptidyl peptidase 4, which is a membrane protease belonging to the serine protease family. DPP4 is widely present in various tissues of the human body, such as the lungs, liver, kidneys, gastrointestinal tract, lymphatic tissue, etc. Its main biological activity is to participate in the metabolism and degradation of peptide hormones, such as glucagon like peptide-1 (GLP-1) and Gastrin releasing peptide (GIP).
The main function of DPP4 is to participate in the metabolic degradation of GLP-1 and GIP. GLP-1 and GIP are two peptide hormones, both of which are important regulators of insulin. GLP-1 can lower blood sugar by activating insulin secretion and inhibiting gastrointestinal motility, while GIP is mainly involved in regulating insulin secretion. However, the life cycles of these two hormones are very short. The half-life of GLP-1 plasma is only 1-2 minutes, while the half-life of GIP is slightly longer, about 7 minutes. Therefore, in order to increase their biological effects in the body, enzymes that degrade these peptide hormones have emerged. As one of the most important degrading enzymes, DPP4 accelerates the metabolism of these two hormones, reduces their active time, and thus reduces their biological activity.
In order to study the biological activity of DPP4, scientists used some activity measurement methods and detection principles. The most commonly used detection method currently is fluorescence substrate dye binding technology. This technology utilizes a high-throughput screening platform to automate sample analysis. Dyes combine with fluorescent groups to generate fluorescent signals and release their own fluorescent labels, thus achieving the basic requirements for activity determination. At the same time, it can also overcome some conventional problems, such as paying attention to sample heterogeneity and mutations.
Another common detection method is HPLC (High Performance Liquid Chromatography). This method is a signal generation and detection method, where the separation and quantification of colorless or colored compounds is a common analytical method. This method can utilize the metabolites in the sample to evaluate the biological activity of DPP4, while also achieving accurate quantification of biological samples.
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