| Cat# | Product Name | Price |
|---|---|---|
| Kit-0300 | DNA Methyltransferase 1 Activity/Inhibitor Screening Assay Core Kit | Inquiry |
| Kit-0301 | DNA Methyltransferase 3B Activity/Inhibitor Screening Assay Core Kit | Inquiry |
| Kit-0302 | DNA Methyltransferase Activity/Inhibition Assay Kit (Colorimetric) | Inquiry |
| Kit-0303 | DNA Methyltransferase Activity/Inhibition Assay Kit (Fluorometric) | Inquiry |
| Kit-0306 | DNMT Activity/Inhibition Colorimetric Assay Ultra Kit | Inquiry |
| Kit-0307 | DNMT Activity/Inhibition Fluorometric Assay Ultra Kit | Inquiry |
| Kit-0308 | DNMT1 Assay Kit | Inquiry |
| Kit-0309 | DNMT3A Assay Kit | Inquiry |
| Kit-0310 | DNMT3B Assay Kit | Inquiry |
| Kit-1714 | DNMT Universal Chemiluminescent Assay Kit | Inquiry |
DNMT (DNA Methyltransferase) is a kind of enzyme that can transfer methyl groups to DNA molecules, thus causing DNA methylation in the genome. This enzyme plays an important role in the human body. Among them, DNMT1 is the "maintainer" of DNA methylation, responsible for transferring methyl to newly synthesized DNA strands during DNA replication and cell division, so as to maintain the stability of cell epigenetic information; DNMT3A and DNMT3B are supplements to the "maintainer" responsible for methylation modifications of new DNA regions. Therefore, it is of great significance to determine and study the activity of DNMTase for further understanding the role of DNA methylation and epigenetic modification.
The main methods for measuring the activity of DNMT enzymes include radiometric assay, non-radiometric assay, and optical assay. Radioassay is one of the earliest methods applied to detect DNMT activity. Its basic principle is to use radiolabeled S-adenosyl-L-methionine (SAM) as a methyl donor and measure the degree of DNA molecular methylation through radioassay. However, the use of radioactive materials in this method will bring about greater security risks and operational risk, and has been gradually replaced in modern laboratories.
Non-radioactive assay can detect DNMT enzyme activity without the use of radioactive substances. The principle is to introduce chemical fluorescence or chemical markers on DNA, and determine the activity of DNMTase by detecting the fluorescence or marker signal changes caused by DNA methylation. This method usually involves multiple steps, such as induction of DNA methylation, DNA purification, DNMT enzyme reaction and post enzyme reaction detection. The operation is complex, but because of its high sensitivity and low technical error, it is widely used in today's research.
Optical assay is a variant of non-radioactive assay, which quantitatively detects the activity of DNMT enzymes through the chemical signals generated by the reaction of specific substrates or active substances with enzymes, such as absorption spectra or fluorescence intensity. This method has the advantages of simple operation, high sensitivity, and short detection time, and is widely used for the determination and research of DNMT enzyme activity in vitro and in vivo.
There are currently various types of DNMT enzyme activity detection kits available on the market for activity determination and screening research of various sample types. These detection kits use various substrates or labeled/fluorescent molecules, and adopt different detection principles to meet the needs and experimental protocols of different researchers.
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