D-Lactate Assay Kit

Common Name :

D-Lactate

Cat.No. :

Kit-0872

Description :

Glyoxalase II converts S-D-Lactoylglutathione and water into D-Lactate from the glyoxalase system pathway. The normal serum concentrations in mammals of D-Lactate are relatively low ranging in the nano to micromolar level. However during a bacterial infection in humans the serum concentration can rise to the millimolar range. High concentrations of D-lactate are indicative of ischemia, trauma or sepsis. The D-Lactate Assay Kit is based on the specifically oxidized D-lactate by D-Lactate dehydrogenase which exhibits an absorbance maximum at 490 nm. The intensity of the absorbance is proportional to the lactate concentration. Using a set of lactate standards, the assay can measure the concentration of lactate in samples (plasma, medium, or others) in a quantitative manner. The kit is stable until the expiration date of under proper storage and handling conditions. The product is for research only.

Applications :

For biological research: D-Lactate measurement in biological samples
For drug/pharm research: Drug influence on D-Lactate metabolism

Storage :

at -80°C

Shipping :

Icepacks

Detection method :

Colorimetric method at 490nm

Compatible Sample Types :

Plasma,Serum,Cell culture supernatant, other body fluid

Sensitivity :

60µM-3000µM