Product Overview :
Cysteine Assay Kit provides a simple, sensitive, and high-throughput adaptable assay that detects physiological concentration of cysteine in a variety of biological fluids. The principle of the assay is based on the cleavage of thiol group of reduced cysteine producing a fluorophore (Ex/Em = 365/450 nm) with a stable signal, which is directly proportional to the amount of total cysteine in the sample. The assay is specific and other thiol-based amino acids do not interfere with the assay. The assay can detect as little as 10 µM of Cysteine in a variety of samples.
Storage :
Store kit at -20°C, protected from light. Briefly spin small vials prior to opening. Read the entire protocol before performing the assay.
Kit Components :
CYS Assay Buffer 25 ml;
Enzyme Mix I 50 µl;
Enzyme Mix II 3 vials;
Reducing Agent 2 vials;
HCY Blocker 100 µl;
CYS Probe 0.5 ml;
CYS Standard 1 vial
Probe; CYS Standard
Compatible Sample Types :
Serum, plasma etc.
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