| Cat# | Product Name | Price |
|---|---|---|
| Kit-0258 | Cytochrome P450 Reductase (CPR) Colorimetric Activity Kit | Inquiry |
| Kit-0924 | CYP1A2 Blue Screening Kit | Inquiry |
| Kit-2126 | Cytochrome P450 1A2 Fluorometric Activity Assay Kit | Inquiry |
| Kit-2127 | Cytochrome P450 1A2 Inhibitor Screening Kit | Inquiry |
| Kit-0923 | CYP2A6 Blue Screening Kit | Inquiry |
| Kit-0917 | CYP2B6 Blue Screening Kit | Inquiry |
| Kit-0908 | CYP2C19 Blue Screening Kit | Inquiry |
| Kit-2124 | Cytochrome P450 2C19 Fluorometric Activity Assay Kit | Inquiry |
| Kit-2125 | Cytochrome P450 2C19 Inhibitor Screening Kit | Inquiry |
| Kit-0895 | CYP2C8 Green Screening Kit | Inquiry |
Cytochrome P450, or CYP for short, is an important enzyme protein located on the endoplasmic reticulum. It widely exists in the liver, intestine, lung and other tissues of human and other organisms, and is a common catalytic enzyme in cells. CYP family plays an extremely important role in many physiological, pharmacological and toxicological processes, and plays a key role in drug metabolism, hormone synthesis and metabolism, energy metabolism and environmental chemicals metabolism.
The biological activity of CYP is mainly manifested in drug metabolism, toxin detoxification, hormone production, and other aspects. Drug metabolism is one of the important biological activities related to CYP. CYP converts drugs into more easily excreted metabolites or new compounds that are more easily absorbed into the body through reactions such as oxidation, reduction, and dehydrogenation, thereby reducing toxic side effects and promoting drug efficacy. Toxin detoxification is another important biological activity of the CYP family. CYP can metabolize foreign toxins in the body, transforming them into more easily excreted metabolites, thereby reducing their toxic effects on the human body. This is very important for the treatment of toxic substances.
The main methods for determining CYP activity include fluorescence probe method, high-performance liquid chromatography, enhanced fluorescence photon plate technology, and CYP450 expression system. The fluorescent probe method is to protonation the spontaneous fluorescent chemicals, and then carry out catalytic oxidation through CYP450 family. Finally, the fluorescent products are separated by chromatography and detected for activity detection. High performance liquid chromatography is also a widely used activity detection method, suitable for low enzyme activity and drug concentration situations. The enhanced fluorescence photon substrate technology exposes fluorescent products to biochemical enzyme labeling instruments and detects them through related measurements of substrate concentration and fluorescence. In the CYP expression system, recombinase technology is commonly used to recombine the CYP gene, transform it into cells to make its gene express and produce living enzymes in the endoplasmic reticulum of cells.
The activity detection principle of CYP is mainly based on the substrate conversion principle and the control experimental principle. The principle of substrate conversion refers to the addition of reaction substrates to a system containing CYP enzymes. After metabolic reactions, the amount of substrate conversion products in the reaction is measured to understand the activity of CYP metabolic reactions. The principle of control experiments is to react substrates without CYP enzymes with test substrates to eliminate the effects of non-specific reactions and non CYP enzymes, thereby more accurately detecting the metabolic reactions of CYP enzymes.
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