COX Fluorescent Inhibitor Screening Assay Kit

ShortName :

COX Fluorescent Inhibitor Screening Assay Kit

Size :

2 x 96 wells

Product Overview :

COX Fluorescent Inhibitor Screening Assay provides a convenient fluorescence-based
method for screening both ovine COX-1 and human recombinant COX-2 for isozymespecific
inhibitors. The assay utilizes the peroxidase component of COXs. The reaction
between PGG2, and ADHP (10-acetyl-3,7-dihydroxyphenoxazine) produces the highly
fluorescent compound resorufin. Resorufin fluorescence can be easily analyzed with
an excitation wavelength of 530-540 nm and an emission wavelength of 585-595 nm.

Storage :

Kit components may be stored at -80°C prior to use. After opening the kit, we
recommend each kit component be stored according to the temperature required.

Shipping :

Dry ice

Kit Components :

Assay Buffer(10X) 2 vials -20°C
Hemin 2 vials -20°C
COX-1(ovine)COX-FIS Assay Reagent 1 vial -80°C
COX-2(human recombinant) COX-FIS Assay Reagent 1 vial -80°C
Arachidonic Acid (substrate) 2 vials -80°C
Potassium Hydroxide 2 vials -20°C
DMSO Assay Reagent 1 vial/3 ml -20°C
ADHP Assay Reagent 6 vials -20°C
DuP-697 Assay Reagent 1 vial -20°C
SC-560 Assay Reagent 1 vial -20°C
96-Well Solid Plate (black) 2 plates RT
Plate Cover 2 covers RT