Product Overview :
AMP Deaminase Assay Kit is the first non-radioactive and continuous assay designed to measure AMP-deaminase content in samples. This enzymatic assay is based on a reaction involving Inosine Monophosphate Dehydrogenase (IMPDH).
(1) In the presence of AMP, AMP Deaminase (AMPD) enzyme catalyzes the formation of IMP;
(2) In the presence of NAD, IMP is immediately oxidized by a highly active IMPDH in the presence of NAD with simultaneous formation of NADH2 directly monitored spectrophotometrically at 340 nm.
The assay is developed for measuring AMP deaminase activity in vitro or in cell lysates. For maximal accuracy, the assays with cell lysates are run with and without AMP substrate in parallel. The absorbance rate observed in the absence of P-ribose is used as blank and is subtracted from the absorbance rate measured in its presence.
Storage :
The kit is shipped at room temperature since reagents and lyophilized enzymes are stable at room temperature up to 2 weeks. However, for long time storage the kit should be frozen upon arrival and stored at -20°C.
Size :
24 assays (8 samples in triplicate)
Kit Components :
Kit Contents (for 10mL of reaction mixture):
Once dissolved, the reagents can be stored at -20°C for three months and used to prepare as much of reaction mixture as needed.
A standard AMPD Assay Kit contains:
• Cysteine (10mg, powder);
• NAD (17mg, powder);
• AMP (12,5mg, powder);
• IMPDH enzyme, lyophilized;
• Reaction buffer (glass vial, 10mL);
• one transparent 96-well plate (round-bottom 96-well plate)
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