Product Overview :
We offer this quick (10 min) HRP assay in a one-step, homogeneous, no wash assay system. This kit uses our fluorogenic Red HRP substrate to quantify peroxidase in solutions. It can be used for ELISAs, characterizing kinetics of enzyme reaction and high throughput screenings, etc. This Fluorimetric Peroxidase Assay Kit provides an optimized "mix and read" assay protocol that is compatible with HTS liquid handling instruments. It can detect as low as 10 uU/mL HRP. The assay can be performed in a convenient 96-well or 384-well microtiter-plate format and easily adapted to automation without a separation step. Its signal can be easily read by either a fluorescence microplate reader at Ex/Em = 540±10/590 ±10 nm (maximum Ex/Em = 540/590 nm) or an absorbance microplate reader at 576±5 nm.
Description :
Horseradish Peroxidase (HRP) is a small molecule (MW ~40 KD) that is widely used in a variety of biological detections. HRP conjugates are extensively used as secondary detection reagents in ELISAs, immunohistochemical techniques; Northern, Southern and Western blot analyses. Due to its small size, it rarely causes steric hindrance problem with the antibody/antigen complex formation. It is usually conjugated to an antibody in a 4:1 ratio. Additionally, HRP is inexpensive compared to other labeling enzymes. The major disadvantage associated with peroxidase is their low tolerance to many preservatives such as sodium azide that inactivates peroxidase activity even at low concentration.
Storage :
Keep in freezer and avoid exposure to light.
Kit Components :
Component A: Red Peroxidase Substrate 1 vial
Component B: H2O2 1 vial (3% stabilized solution, 200 μL)
Component C: Assay Buffer 1 bottle (100 mL)
Component D: Horseradish Peroxidase 1 vial (20 units)
Component E: DMSO 1 vial (1 mL)
Features & Benefits :
Broad Application: Can be used for quantifying HRP activities in solutions and solid surfaces (e.g, ELISA)
Sensitive: Detect as low as 10 uU/mL HRP in solution.
Continuous: Easily adapted to automation without a separation step.
Convenient: Formulated to have minimal hands-on time. No wash is required.
Non-Radioactive: No special requirements for waste treatment.
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