Product Overview :
Citrate Assay Kit is a quantitative colorimetric/fluorimetric determination of citrate at OD570, FL530/585 nm.
Description :
CITRATE is an intermediate in the citric acid cycle and is involved in fatty acid synthesis. Citrate Assay Kit provides a simple, and automation-ready procedure for measuring citrate concentration. Citrate is converted into pyruvate which is then oxidized with the conversion of the dye into a colored and fluorescent form. The color intensity at 570 nm or fluorescence intensity at λex/em = 530/585 nm is directly proportional to the citrate concentration in the sample.
Applications :
Citrate determination in biological samples (e.g. plasma, serum, urine, tissue and culture media.)
Usage :
For research use only (RUO)
Storage :
Store all kit components at -20°C. Shelf life of six months after receipt.
Kit Components :
Developer 10 mLDye Reagent 120 µLCitrate Standard 500 µLCL Enzyme DriedODC Enzyme 120 µL
Detection method :
Colorimetric, Fluorimetric
Compatible Sample Types :
Culture Medium, Plasma, Serum, Tissue, Urine
Features & Benefits :
Fast and sensitive. Linear detection range: 4 to 400 µM citrate for colorimetric assays and 0.5 to 40 µM for fluorimetric assays.Convenient and high-throughput. Homogeneous "mix-incubate- measure" type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day.
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