Product Overview :
Cholesterol Assay Kit is a fluorescence-labeled method for quantification of cholesterol.
Description :
Cholesterol circulates in the blood as a free acid, as well as, esterified to long-chain fatty acids called cholesteryl esters. Cholesteryl esters are the preferred form for cholesterol transport and storage. Lecithin: cholesterol acyltransferase (LCAT) is a 67-kDa glycoprotein that is responsible for cholesterol esterification in plasma. The enzyme displays two activities: a phospholipase A2 activity, which hydrolyzes the fatty acyl group from the sn-2 position of phosphatidylcholine; and a transacylase activity, which catalyzes the transfer of the fatty acyl group from the acyl-enzyme complex to the 3-β hydroxyl group of cholesterol to form cholesteryl ester. Elevated levels of cholesterol and cholesteryl esters have been linked to atherosclerosis and heart disease. This has resulted in a large amount of research focused in the understanding of cholesterol homeostasis. Quantitation of cholesterol in experimental samples is imperative to this research.
Usage :
For research use only (RUO)
Storage :
This kit will perform as specified if stored at -20°C and used before the expiration date indicated on the outside of the box.
Kit Components :
Cholesterol Assay Buffer (10X) 3 mLCholesterol Assay Standard 100 μLCholesterol Assay Detector 2 vialsCholesterol Assay Horseradish Peroxidase 1 vialCholesterol Assay Oxidase 1 vialCholesterol Assay Esterase 1 vialDMSO Assay Reagent 1 mL96-Well Solid Plate (black) 1 plate96-Well Cover Sheet 1 cover
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