Product Overview :
Cell-based Cholesterol Efflux Assay Kit (Fluorometric) is a high-throughput screening assay for measuring cholesterol efflux.
Description :
Cholesterol efflux from the peripheral tissues and cells in atherosclerotic plaque is an initial and critical step in Reverse Cholesterol Transport (RCT). RCT is the process by which extrahepatic cells, including macrophage-derived foam cells in arterial atherosclerotic plaque, transport excessive cholesterol back to the liver for bile acid synthesis and excretion, thus lowering the peripheral lipid burden. A negative correlation has been established between the in vitro efflux of cholesterol from macrophages and atherosclerosis. The Cell-based Cholesterol Efflux Assay Kit (Fluorometric) is a high-throughput screening assay for measuring cholesterol efflux in cells using fluorescently-labeled cholesterol. This assay provides a safe, sensitive, and reproducible method for measuring cholesterol efflux.
Applications :
Screen serum samples or lipoproteins (isolated or recombinant) for cholesterol efflux.Screen small molecules for their effect on cholesterol efflux (a valuable tool for drug discovery program).
Usage :
For research use only (RUO)
Storage :
Store the kit at -20°C, protected from light.
Kit Components :
Labeling Reagent 5 mLEquilibration Buffer 5 mLReagent A 10 μLReagent B 10 μLCell Lysis Buffer 20 mLPositive Control 1 mLSerum Treatment Reagent 1 mL
Detection method :
Fluorometric
Compatible Sample Types :
Isolated lipoproteins, Recombinant lipoproteins, Serum
PDF