Cathepsin L Activity Fluorometric Assay Kit

Product Overview :

Cathepsin L Activity Assay Kit is a homogeneous assay that can be used to detect the activity of enzyme in biological samples or in purified enzyme preparations. The long wavelength FRET substrate is designed based on a sequence surrounding the cleavage site of Cathepsin L. A Fluor 488 pair is used for optimal quenching of the intact substrate. When active Cathepsin L cleaves the FRET substrate, it results in an increase of Fluor 488 fluorescence and can be monitored at excitation/emission = 490 nm/520 nm. The fluorescent signal from Fluor 488 is stable at low pH, the optimal pH for Cathepsin L activity.

Size :

100 Assays

Storage :

Store component C at -80°C. Store all other components at -20°C. Component D can be stored at room temperature for convenience. Protect components A and B from light and moisture.

Shipping :

Dry ice

Kit Components :

Component A: Cathepsin L substrate, Ex/Em=490 nm/520 nm upon cleavage: 1 mM, 50 µL
Component B: Fluorescence reference standard, Ex/Em=490 nm/520 nm: 1 mM, 10 µL
Component C: Cathepsin L, human recombinant: 0.1 mg/mL, 20 µL
Component D: Assay Buffer: 20 mL
Component E: Cathepsin L inhibitor: 100 µM, 10 µL
Component F: DTT: 1 M, 200 µL