What is a Catalase

Catalase is an important enzyme protein that mainly exists in the cells of organisms and is an oxidoreductase. In cells, its function is to convert hydrogen peroxide in metabolites into water and oxygen. The biological function of Catalase is very important because its existence can effectively maintain metabolic balance within cells and maintain the stability of the internal and external environment of organisms. Therefore, the activity of Catalase can be used as an indicator to evaluate the metabolic status of organisms.

The Biological Activity of Catalase

Catalase is not only an oxidoreductase, but also a catalytic enzyme that can promote the progress of chemical reactions. In cells, the main function of Catalase is to convert hydrogen peroxide (H2O2) into water (H2O) and oxygen (O2), which is an important oxidation reaction. Therefore, Catalase is also known as H2O2 enzyme. Catalase plays an important role in cellular redox metabolism.

Catalase activity determination method and detection principle

The method for measuring the activity of Catalase is relatively simple, and its activity can be evaluated by observing the speed of enzyme catalyzed reactions. The commonly used measurement methods include measuring the rate of oxygen generation, measuring the rate of hydrogen peroxide consumption, and measuring the rate of sodium selenite consumption. One of the most common methods is to determine the rate of hydrogen peroxide consumption.

The specific operation is as follows: add phenol red as an indicator to the sample, and then add hydrogen peroxide as the substrate. After adding the substrate, inject a certain amount of catalyst Catalyst, mix the reaction well, and measure the generated oxygen volume or hydrogen peroxide consumption within a certain time (usually 30-60 seconds). Evaluate the activity of Catalyst based on its amount. Catalase has high activity, which can convert more hydrogen peroxide, generate more oxygen, and even generate bubbles. The oxygen volume is also relatively large, indicating that Catalase has high activity.

Another fluorescence detection kit utilizes the catalase enzyme catalyzed reaction of H2O2 decomposition into two products, water and oxygen, to detect the activity of the catalase enzyme in the sample. This detection method involves two key reagents: one is H2O2; Another type is chromosome or fluorometric.

After the reaction begins, the Catalase enzyme in the sample catalyzes the decomposition of H2O2, producing two signaling substances: oxygen and chromosomes or fluorescein. The color or fluorescence of chromosomes or fluorescein increases with the increase of Catalase activity in the sample, so the activity of Catalase can be quantitatively measured by measuring changes in chromosome color or fluorescence.

SUBMIT