Product Overview :
The Fluorimetric Catalase Assay Kit provides a quick and sensitive method for the measurement of catalase activity. It can be performed in a convenient 96-well or 384-well microtiter-plate format and easily adapted to automation without a separation step. Catalase reacts with H2O2 to produce water and oxygen (O2). CM Red also reacts with H2O2 to generate a red fluorescent product. Therefore the reduction in fluorescence intensity is proportional to catalase activity. The CM Red substrate used in the assay enables a dual recordable mode. The fluorescent signal can be easily read by either a fluorescence microplate reader or an absorbance microplate reader. With the Fluorimetric Catalase Assay Kit, we have detected as little as 30 mU/mL catalase in a 100 µL reaction volume.
Warning :
Thaw all the kit components at room temperature before starting the experiment. The component A is unstable in the presence of thiols such as DTT and β-mercaptoethanol. The final concentration of the thiols higher than 10 µM would significantly decrease the assay dynamic range. NADH and glutathione (reduced form: GSH) may interfere with the assay.
Kit Components :
Component A: CM Red Freeze (< -15 °C), Minimize light exposure 1 vial
Component B: H2O2, Freeze (< -15 °C), Minimize light exposure, 1 vial (3% stabilized solution, 200 µL)
Component C: Assay Buffer, Refrigerated (2-8 °C), 1 bottle (50 mL)
Component D: Horseradish Peroxidase, Freeze (< -15 °C), Minimize light exposure, 1 vial (20 units)
Component E: Catalase Standard, Freeze (< -15 °C), Minimize light exposure, 1 vial (1000 U/mL, 50 µL)
Component F: DMSO, Freeze (< -15 °C), 1 vial (200 µL)
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