Description :
Activation of ICE-family proteases/caspases initiates apoptosis in mammalian cells. The Caspase-6 Colorimetric Assay Kit provides a simple and convenient means for assaying the activity of caspases that recognize the sequence VEID. The assay is based on spectrophotometric detection of the chromophore p-nitroanilide (pNA) after cleavage from the labeled substrate VEID-pNA. The pNA light emission can be quantified using a spectrophotometer or a microtiter plate reader at 400-or 405-nm.
Storage :
Store kit at –20°C (Store Cell Lysis Buffer, 2X Reaction Buffer, and Dilution Buffer at 4°C after opening). All reagents are stable for at least 6 months.
Handling :
Aliquot enough 2X Reaction Buffer for the number of assays to be performed. Add DTT to the 2X Reaction Buffer immediately before use (10 mM final concentration: add 10 µl of 1.0 M DTT stock per 1 ml of 2X Reaction Buffer). •
After thawing, store Cell Lysis Buffer, 2X Reaction Buffer, and Dilution Buffer at 4°C. •
Protect VEID-pNA from light.
Kit Components :
25ml Cell Lysis Buffer,
2ml 2X Reaction Buffer,
125µl VEID-pNA (4 mM),
100µl DTT (1M),
25ml Dilution Buffer.
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