Product Overview :
The Caspase-2 Fluorometric Assay Kit provides a simple and convenient means for assaying the activity of caspases that recognize the sequence VDVAD. The assay is based on detection of cleavage of substrate VDVAD-AFC (AFC: 7-amino-4-trifluoromethyl coumarin). VDVAD-AFC emits blue light (λmax = 400 nm); upon cleavage of the substrate by caspase-2 or related caspases, free AFC emits a yellow-green fluorescence (λmax = 505 nm), which can be quantified using a fluorometer or fluorecence microtiter plate reader. Comparison of the fluorescence of AFC from an apoptotic sample with an uninduced control allows determination of the fold increase in Caspase-2 activity.
Kit Components :
25ml Cell Lysis Buffer,
2ml 2X Reaction Buffer,
125µl VDVAD-AFC Substrate (1mM),
100µl DTT.
Target Species :
Mammalian
Detection method :
Fluorecence (400 nm excitation filter and 505 nm emission filter)
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