Description :
BCA Protein Assay Kit is a ready-to-use detergent-compatible Western blot related total protein analysis reagent used for the quick determination of total protein concentration by measuring absorbance at 562 nm and comparing to a protein standard absorption vs. concentration curve, according to Smith.
Applications :
Western blotting, protein expression assays, protein profiling and characterization, protein quantitation assays
Usage :
Studying protein-protein interactions, Measuring column fractions after affinity chromatography, Assessing protein yields in whole cell lysates, High-throughput screening of fusion proteins
Storage :
Store at room temperature for one year.
Size :
Tube procedure: 50 assays
Microplate procedure: 500 assays
Kit Components :
BCA Reagent A: 100 ml
BCA Reagent B: 5 ml
Albumin (BSA) Standards: 20 ml (2 mg/ml)
Detection method :
Colorimetric/Spectroscopic
Compatible Sample Types :
Serum, Plasma, Cell culture extracts, Tissue Extracts
Sensitivity :
20 - 2000 μg/ml
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