Auto-ubiquitinylation kit

Cat.No. :

Kit-2589

Product Overview :

This E3 ligase auto-ubiquitinylation kit enables proteins to be tested for ubiquitin E3 ligase activity through assessment of their ability to undergo auto-ubiquitinylation. Utilizing the first three steps in the ubiquitin cascade the kit facilitates ubiquitinylation of known or putative E3 ligase enzymes followed by Western blot analysis using the highly sensitive reagents provided or using antibodies to the specific protein of interest (user supplied). A high integrity ubiquitin E3 ligase enzyme is also provided for use as a positive control. The Kit provides sufficient material for approximately 10 autoubiquitinylation assays.

Applications :

1. Qualitative assessment of an Ub E3 ligase enzyme’s activity through its ability to auto-ubiquitinylate.
2. Testing of proteins for auto-ubiquitinylation activity allowing their identification as putative ubiquitin E3 ligases.
3. Ubiquitinylation of substrate proteins (user provided) specific to a particular ubiquitin E3 ligase.

Storage :

-80°C

Shipping :

Shipped on Dry Ice

Kit Components :

20X Ubiquitin Activating Enzyme Solution (E1), 25µl
20X Ubiquitin Conjugating Enzyme Solution (E2), 25µl
10X Ubiquitin Solution (Ub), 50µl
20X Control Ubiquitin Ligase Solution (E3), 25µl
20X Mg-ATP Solution, 25µl
10X Ub E3 ligase Buffer, 50µl
Ubiquitin antibody solution, 25µl