Description :
ATPase (Adenosine Triphosphatase: EC 3.6.1.3) is an important enzyme for maintaining the cell membrane potential, transporting ions and regulating cellular volume. It catalyzes the decomposition of ATP into ADP and a free phosphate ion. The hydrolysis of ATP is highly exergonic releasing energy that is utilized in several cellular processes. There are many classes of ATPases including Na+/K+ -ATPase, H+/K+ -ATPase, Ca2+ -ATPase, etc. The deficiency of mitochondrial ATPase is serious: for example, Na+/K+-ATPase deficiency increases anxiety-related behavior, while Ca2+ -ATPase deficiency leads to exertional muscle pain syndrome. Therefore, accurate detection of ATPase activity is valuable for the diagnosis and mechanistic studies of some of these diseases. ATPase Activity Assay kit provides a quick and easy method for monitoring ATPase activity in various samples. In the assay, ATPase hydrolyzes ATP releasing ADP and a free phosphate ion, and through linked reactions, a strong, stable chromophore is generated (OD 650 nm). The assay is simple, sensitive, high -throughput adaptable and can detect ATPase Activity less than 0.005 U/L.
Applications :
Measurement of ATPase activity in various tissues/cells
Analysis of energy-generating pathways
Analysis of Na+/K+ -ATPase mediated signal transduction pathways, e.g. MAPK, ROS etc.
Storage :
Store kit at -20ºC, protected from light. Centrifuge vials briefly, prior to use.
ATPase Assay Buffer: Warm to room temperature before use. Store at 4ºC.
ATPase Substrate: Reconstitute one well with 110 µl dH2O. Pipette up and down to dissolve. Aliquot and store at -20°C. Use within two months.
ATPase Developer: Ready to use as supplied and keep it at room temperature.
ATPase Positive Control: Reconstitute with 100 μl Assay Buffer and mix thoroughly. Keep on ice while in use. Aliquot and store at –20°C. Use within two months.
Kit Components :
• ATPase Assay Buffer, 25 ml
• ATPase Substrate, 2 vials
• ATPase Developer, 3 ml
• Phosphate Standard (10 mM), 0.5 ml
• ATPase Positive Control, 1 vial
Compatible Sample Types :
Animal tissues: Liver, heart, kidney, etc.
Cell culture: Adherent or Suspension Cells
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