Product Overview :
ATP synthase (EC 3.6.3.14) is an important enzyme that creates the energy storage molecule adenosine triphosphate (ATP). ATP is the most commonly used "energy currency" of cells for most organisms. It is formed from adenosine diphosphate (ADP) and inorganic phosphate (Pi), and needs energy for its formation. The assay is used to determine Cytochrome C oxidase activity. The enzyme catalysed reaction products Pi can react with dry reagent, and can be measured at a colorimetric readout at 660 nm.
Kit Components :
96-Well Microplate, 1 plate
Assay Buffer I, 30 ml x 4, 4 °C
Assay Buffer II, Powder x 1, 4 °C
Assay Buffer III, 20 ml x 1, 4 °C
Reaction Buffer, 4 ml x 1, 4 °C
Substrate, Powder x 1, -20 °C
Stop Solution, 5 ml x 1, 4 °C
Dye Reagent I, Powder x 1, 4 °C
Dye Reagent II, Powder x 1, 4 °C
Dye Reagent III, 15 ml x 1, 4 °C
Standard (10 μmol/ml), 1 ml x 1, 4 °C
Plate Adhesive Strips, 3 Strips
Compatible Sample Types :
Tissue extracts, Cell lysate, Cell culture media and Other biological fluids Samples
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