| Cat# | Product Name | Price |
|---|---|---|
| Kit-0111 | ATF2 (pT69/pT71) Transcription Factor Assay Kit | Inquiry |
ATF2, an acronym for 'Activating Transcription Factor 2', is a protein encoded in the human body by the ATF2 gene. This protein belongs to the ATF/CREB family of transcription factors and plays an integral role in normal cell physiology and response to environmental stress. It is found in various cell types and tissue components, with transcriptional activation mediated by multiple signaling pathways including that of JNK (c-Jun N-terminal kinases).
ATF2 involves DNA binding capability that can stimulate or repress the gene expression transcription. Alterations or irregular expression in ATF2 has been linked to numerous cancers, including melanoma, making its detection crucial in medical and pathology laboratories.
So, how is ATF2 detected? There are several methods for ATF2 detection which are adopted by researchers and scientists, making them fundamental in our understanding of various pathologies.
Immunoblotting, also known as Western Blotting, is one of the commonly used methods for ATF2 detection. It is a technique that leverages antibodies to detect specific proteins in a given sample. The method begins with the separation of proteins in a sample according to their molecular weight by gel electrophoresis. The proteins are then transferred to a membrane, where they are tagged with an antibody specific to ATF2. Through several washing stages, the specifically bound antibodies, i.e., those attached to ATF2, are visualized, allowing researchers to observe ATF2's presence.
Immunohistochemistry (IHC) is another method used frequently for ATF2 detection. IHC is a popular laboratory method that involves using antibodies to detect specific antigens (in this case, ATF2) in biological tissues. This technique provides anatomical information about protein expression by producing colored deposits where the relevant antigens are present. Once an ATF2-specific antibody is added, it binds to ATF2 in the biological sample. These antibody-antigen complexes are then revealed by chemical reactions resulting in observable color changes.
Other detection methods include immunofluorescence, a technique that utilizes the specificity of antibodies for ATF2 combined with the sensitivity of fluorescent dyes, and DNA-binding enzyme-linked immunosorbent assay (ELISA), a plate-based assay technique designed for detecting and quantifying substances such as peptides, proteins, antibodies, and hormones, including ATF2.
In the scientific marketplace, several ATF2 activity/detection kits are available, offering accurate, innovative, and reliable solutions for ATF2 research. These tools come with comprehensive instructions and necessary components for successful ATF2 activity analysis or detection.
One such kit is the ATF2 (phospho T71) in-vitro ELISA kit. It is designed for the quantitative measurement of ATF2 protein in human cell extracts.
Another ATF2 detection kit that stands out is Total ATF-2 Sandwich ELISA Kit. It is a solid-phase sandwich ELISA that detects endogenous levels of total ATF-2 protein.
These kits built around the principles of ELISA, provide detailed protocols and standards for generating results, allowing for a simpler, more accessible process of ATF2 detection.
In summary, ATF2, an essential protein with significant role in transcriptional regulation, response to environmental stress and several pathological conditions, can be accurately detected using a variety of innovative and reliable techniques. Immunoblotting, IHC, immunofluorescence, and ELISA methods provide reliable ways to uncover the presence and quantify ATF2 in biological samples. Several commercial ATF2 activity/detection kits provide further support for these endeavours, bringing precision, reliability, and accessibility to ATF2 research.
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