| Cat# | Product Name | Price |
|---|---|---|
| Kit-1683 | ATAD2B Inhibitor Screening Assay Kit | Inquiry |
| Kit-1684 | ATAD2B TR-FRET Assay Kit | Inquiry |
Human biology entails a wide range of elements associated closely with our health. One such crucial element is the gene. In the vast labyrinth of genes that create the blueprint of our bodies, one name which has garnered significant attention is ATAD2B (ATPase Family, AAA Domain Containing 2B).
ATAD2B is a gene that encodes the protein ATAD2B. Primarily, genes play a crucial role in the making of proteins. The ATAD2B gene is recognized as part of the ATPases Associated with various cellular Activities (AAA) family. This family includes ATP (adenosine triphosphate)-dependent chromatin-remodeling enzymes. The AAA proteins are involved in multifarious cellular functions, including cell cycle progression, DNA replication, and protein degradation.
The proteins encoded by ATAD2B possess an AAA domain, which is key for ATPase activity, and a bromodomain. ATPases are enzymes that break down the molecule ATP, releasing a substantial amount of energy utilised by the cells. The bromodomain enables these proteins to bind to acetylated histone proteins, facilitating chromatin remodeling.
The detection of ATAD2B should be prioritized because aberrant expression can potentially lead to chromosomal instability and subsequent development of various diseases, including neurodevelopmental disorders and certain types of cancers. Specific methods have been developed to detect the presence and study the activity of the ATAD2B gene and its coded proteins.
A common method for detecting ATAD2B and analyzing its gene expression is through quantitative real-time polymerase chain reaction (qRT-PCR). This sensitive and precise technique allows researchers to detect and measure the amount of specific genetic material in a sample.
Western blotting can be utilised to detect ATAD2B proteins. This method relies on detecting proteins in a sample through their molecular weight and charge. The proteins of interest are separated using gel electrophoresis and transferred onto a membrane, which is then probed with specific antibodies against the protein.
Immunohistochemistry (IHC) is another method used for ATAD2B detection. This technique uses antibodies that bind to specific antigens in a tissue sample, enabling their visualisation using a microscope.
Every ATAD2B detection method works on the principle of DNA or protein interaction with specific probes or antibodies. These methods are designed to provide information on the expression level, protein function and interaction, and cellular location.
There are several kits available that assist in the detection of ATAD2B. Some of these include:
ATAD2B Human ELISA Kit: This enzyme-linked immunosorbent assay (ELISA) kit is designed for the quantitative measurement of Human ATAD2B in cell culture supernatants, cell lysates, serum, and plasma.
Human ATAD2B ELISA Kit: This kit includes biotinylated anti-Human ATAD2B antibody, Avidin-conjugated Horseradish Peroxidase (HRP), standard recombinant human ATAD2B protein, and other necessary components for specific, sensitive, and quantitative detection of natural and recombinant Human ATAD2B.
In the realm of biotechnology, these ingenious tools offer a vista of possibilities, enabling scientists and researchers to understand the nature, function, and role of ATAD2B gene much better. Their critical findings, in turn, contribute to knowledge, growth, and innovation in medical and biological sciences, prompting advancements and improvements in health and disease management.
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