| Cat# | Product Name | Price |
|---|---|---|
| Kit-1681 | ATAD2A Inhibitor Screening Assay Kit | Inquiry |
| Kit-1682 | ATAD2A TR-FRET Assay Kit | Inquiry |
The rapid progress in the field of biological sciences has led to the discovery and understanding of myriad genes and proteins that play pivotal roles in the maintenance of life. One such protein that has gained attention in recent years is ATAD2A (ATPase Family AAA Domain-Containing Protein 2A), a member of the ATPase superfamily.
ATPases, or ATP synthases, are enzymes that catalyze the decomposition of ATP (Adenosine Tri-Phosphate) into ADP (Adenosine Di-Phosphate) and an inorganic phosphate. This reaction releases energy used in several biological functions, ranging from ion movement to contraction of muscles. The ATPase Family AAA Domain-Containing Protein 2A, known as ATAD2A, is one such ATPase.
Detailed research on ATAD2A shows that it is a co-factor protein that plays a crucial role in oncogenic transcription, suggesting that it has a key role to play in various cancers. Notably, high levels of ATAD2A have been identified in prostate cancer, breast cancer, endometrial cancer, and thyroid cancer, to name a few. It has been associated with more aggressive tumor phenotypes, poorer prognosis, and resistance to specific treatments, establishing ATAD2A as an exciting prospect for cancer research and therapeutics.
Given the importance of ATAD2A in cancer research and understanding the disease, scientists have developed different methodologies to detect and quantify the levels of this protein in cells.
Immunohistochemistry (IHC) is one of the commonly used methods for detection. IHC involves staining the protein using specific antibodies developed against ATAD2A, thus allowing researchers to visualize its location within cells and tissues.
Another common method for ATAD2A detection is Western Blotting. This technique separates proteins based on their sizes using electrophoresis, transfers them onto a membrane, and uses antibodies that bind specifically to ATAD2A. The bound antibodies can then be detected using appropriate secondary antibodies linked to an enzyme, leading to colour development or chemiluminescence, which correlates with the ATAD2A protein level.
Quantitative real-time polymerase chain reaction (qRT-PCR) is another accurate and reliable method used for determining the levels of ATAD2A in various samples. RNA is extracted from the samples, reverse transcribed into complementary DNA (cDNA), and then specific sequences of ATAD2A are amplified and quantified. The amount of product generated correlates with the initial amount of the specific cDNA, enabling ATAD2A quantification.
Due to the vital role of ATAD2A in cancer biology and the need for its detection in research, numerous kit systems have been developed to simplify this process.
The ATAD2A Human ELISA Kit is an example. This detection kit is designed for the quantitative measurement of Human ATAD2A in cell culture supernatants, cell lysates, serum, and plasma. It includes components for a solid phase sandwich ELISA assisted by two antibodies binding to ATAD2A.
In summary, ATAD2A is a crucial protein largely implicated in various cancer pathogeneses, offering vast potential for the future of cancer research and therapeutics. Timely detection of ATAD2A plays a central role in cancer diagnosis and understanding the disease progression. The ready availability of detection kits and methodologies brings us a step closer to uncovering the untapped potential held within the tiny structure of ATAD2A.
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