| Cat# | Product Name | Price |
|---|---|---|
| Kit-2453 | Aspartate Transaminase Assay Kit | Inquiry |
| Kit-0110 | Aspartate Transaminase Assay Kit | Inquiry |
| Kit-2303 | Aspartate Aminotransferase (AST or SGOT) Activity Colorimetric Assay Kit | Inquiry |
The world of biochemistry galore with a myriad of enzymes, each having an essential function in the overall metabolism and physiological processes of the organism. Among these enzymes is one that goes by the name of Aspartate Transaminase (AST), also known as GOT (Glutamic-Oxaloacetic Transaminase).
Starting with the basic, what exactly is Aspartate Transaminase? It is an enzyme that catalyzes the interconversion of aspartate and alpha-ketoglutarate to oxaloacetate and glutamate. This reaction is an important one, forming part of the amino acid metabolism pathway. AST is primarily found in cells of the heart and liver, but also to a lesser extent in skeletal muscles and kidneys. This cellular residency of the enzyme also has diagnostic implications, linking the raised levels of AST with liver diseases including hepatitis and cirrhosis, as well as cardiovascular problems like myocardial infarction amongst others.
The scientific and medical interest in AST involves not just knowing what it is and where it's found, but possessing strategies to accurately detect and measure its levels. This brings us to the various methods and principles involved in Aspartate Transaminase detection.
One of the most frequently used methods for AST detection is the Colorimetric method, which involves the conversion of NADH to NAD and the subsequent decrease in absorbance observed at 340 nm. An alternative method includes the UV Kinetic method, which majorly relies on the same reaction but measures the increase in NADH.
Another commonly used method is the IFCC method. This method uses pyridoxal-5'-phosphate (P-5'-P) as a coenzyme and L-aspartate as a substrate to observe AST activity. Alanine Transaminase (ALT) is measured at the same time to produce a ratio of the two enzymes (AST/ALT ratio), which is a useful diagnostic factor in liver disease investigations.
In addition to these, a host of biosensor-based methods have been developed for AST detection, taking advantage of recent advancements in nanotechnology. These include graphene-based biosensors and electrochemical biosensors that offer the advantages of high sensitivity and selectivity.
For researchers who need to analyze samples for AST activity, many commercially available kits allow for efficient and reliable detection. Creative BioMart offers an Aspartate Transaminase Activity Assay Kit, designed to measure AST activity in biological samples such as serum, plasma, and other cell lysates. The kit uses a straightforward colorimetric assay that can be performed in a common spectrophotometer.
Other notable kits in the market include Aspartate Transaminase Activity Colorimetric Assay Kit that provides a simple and direct procedure for measuring AST activity in a variety of samples, including serum, plasma and tissue homogenates.
In summary, Aspartate Transaminase is an essential enzyme, abundant in the liver and heart, which plays a significant role in amino acid metabolism. Its activity contributes to our understanding of certain diseases and can be detected using various methods and commercially available kits. These detection tools, based on complex principles, make it possible to discern the AST level in various samples, contributing vitally to diagnoses and treatment strategies.
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