Product Overview :
Ascorbic acid (the L-enantiomer commonly known as vitamin C) is an important antioxidant found in living organisms and applied as additives in food and other industrial processes. By reacting with reactive oxygen species, it protects the cell from oxidative damages. Kit-0105 provides a simple, direct and high-throughput assay for measuring ascorbic acid. In this assay, ascorbic acid is oxidized by ascorbate oxidase resulting in the production of H2O2 which reacts with a specific dye to form a pink colored product. The color intensity at 570nm or fluorescence intensity (530/585 nm) is directly proportional to the ascorbic acid concentration in the sample.
Applications :
Assays: ascorbic acid in biological samples such as serum, plasma, urine, saliva, milk, tissue, and cell culture.
Drug Discovery/Pharmacology: effects of drugs on ascorbic acid metabolism.
Storage :
The kit is shipped on ice. Store all components at -20°C. Shelf life of 12 months after receipt.
Kit Components :
Assay Buffer: 10 mL
Enzyme Mix: 120 µL
Dye Reagent: 120 µL
Standard: 400 µL 10 mM ascorbic acid
Detection method :
OD570nm, or FL530/585nm
Compatible Sample Types :
Serum, plasma, urine, saliva, milk, tissue, and cell culture
Features & Benefits :
Use 20 μL samples. Linear detection range: colorimetric assay 6 to 1000 μM, fluorimetric assay 1 to 100 μM ascorbic acid.
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