| Cat# | Product Name | Price |
|---|---|---|
| Kit-0103 | Arginase Assay Kit | Inquiry |
| Kit-2259 | Arginase Activity Colorimetric Assay Kit | Inquiry |
| Kit-2474 | Arginase Assay Kit | Inquiry |
Arginase is a distinct enzyme that plays a crucial role in the metabolism of nitrogen as it catalyzes the detoxification of ammonia to urea in the liver, as part of the urea cycle. The enzyme has two isoforms: Arginase 1 and 2, both belonging to a family of binuclear manganese metalloenzymes.
Arginase 1 is deemed to be a cytosolic enzyme principally located in the liver, while Arginase 2 resides in the mitochondria and is ubiquitously expressed in tissue types rather than the liver. Throughout the years, studies have demonstrated that both forms of Arginase are significant within the immune system controlling the activation, proliferation, and survival of the body's defense mechanism.
In humans, defects in Arginase 1 gene could result in argininemia, a rare inherited metabolic disorder whereby the body can't effectively dispel excess nitrogen. This condition, often diagnosed during infancy, may lead to intellectual and growth retardation, vomiting, irritability, lack of muscle coordination, seizures, among other devastating downfalls.
Measuring Arginase activity is essential in discovering various ailments, including liver diseases, allergic reactions, autoimmune diseases, and certain cancers. Many different detection methods are available, each operating under different principles.
One principle includes spectrophotometry, a common approach which often involves measuring the production of urea. The basic principles for this detection method are centered on the Arginase enzyme converting L-Arginine to L-Ornithine and urea. The concentration of urea can be indirectly measured through relevant reaction steps subsequently derived. Another method involves the detection of ornithine using o-phthalaldehyde (OPA) under alkaline conditions and spectrophotometric analysis.
An alternative detection principle is centered on the fluorescence spectrophotometry method. This technique also works by the same enzymatic reactions as for the spectrophotometry method. The produced ornithine is then derivatized with OPA, but in this case, OPA-ornithine becomes highly fluorescent upon excitation, therefore, the ornithine becomes detectable.
In addition, a variety of bioassays are available for measuring Arginase activity. Bioassay methods often involve evaluating an enzyme's performance by conducting a biological measurement of its function within the body. The assay operates by detecting the ability of the body's immune system to accurately react against Arginase's enzyme activity.
Numerous commercially available kits are designed to facilitate the detection and measurement of Arginase enzyme activity. These kits are typically designed based on the different principles and methods discussed previously.
While Arginase's role within our biological systems is paramount, so is our understanding of its functional levels. Through utilizing a variety of detection methods, scientists continue to make advancements in deciphering Arginase's fundamental role in metabolic and immune pathways.
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