Application of Assay Kit

The assay kit is used to determine serum, plasma, and related fluids. For example, it is suitable for testing samples such as urine, pleural and ascites fluid, lavage fluid, cell culture supernatant, and tissue homogenate. The assay kit has gradually become an essential tool in scientific research. Our company provides various products with reliable quality, preferential prices, high sensitivity, stable effect, easy storage, and simple operation.

The technical principles of the assay kit are as follows:

1. Immobilization and enzyme labeling of antigen or antibody.
2. The antigen or antibody bound to the surface of the solid carrier still maintains its immunological activity.
3. Enzyme-labeled antigen or antibody retains both its immunological activity and enzyme activity.
4. The test specimen reacts with the antigen or antibody on the surface of the solid phase carrier. After adding an enzyme-labeled antigen or antibody, it is also bound to the solid-phase carrier through the reaction.
5. The amount of enzyme on the solid phase is proportional to the tested substance in the specimen.
6. After added into enzyme reaction, the substrate is catalyzed and turns into a colored product. The product amount is directly related to the substance in the specimen. They can be qualified according to the degree of color. The measurement method has high sensitivity and good repeatability. It is based on the immunological reaction, a highly sensitive test technique combining antigen-antibody reactions with the efficient catalytic action of enzymes on the substrate.

In scientific research, the main applications of assay kits are enzyme activity assays, metabolism and endocrinology assays, epigenetic assays, cellular and biochemical assays, cell signaling assays, detection assays, etc.

• Enzyme Activity Assays

Enzyme activity assay is an analytical technique that uses the properties of enzymes to specifically and efficiently catalyze chemical reactions and detects the content and activity of certain enzymes in biological samples. The enzyme activity assay kit provides a powerful and convenient way to measure enzyme activity in a sample.

The reagent contains a substrate. If your sample contains an enzyme, it will react with the substrate in the reagent. The amount of substrate produced is detected by absorption, and the enzyme activity can be tested through this reaction.

• Metabolism and endocrinology assays

Metabolism is a complex process and the core of biology. In metabolism and endocrinology research, we need to detect and quantify various circulating biomarkers in a highly sensitive, specific, and reliable manner. Detection kits for the study of metabolism and endocrinology allow researchers to quickly obtain a large number of sample-detection data from a single experiment.

• Epigenetic Assays

Epigenetic regulation occurs at the level of many interactions. Multiple epigenetic factors work together to coordinate the fundamental cellular processes ranging from development to cell death. To understand all aspects of biology or diseases, scientists need to observe all these levels in parallel to understand the role of epigenetics in promoting biological processes. Different types of assay kits help researchers better conduct epigenetic research.

• Cellular and Biochemical Assays

Cells are the basic structural and functional units of organisms as a starting point to determine and map the basic biochemical and physical processes of life. Cellular and biochemical assays are important tools for life science research, and they play an increasingly important role in biomedicine research, biochemical product generation, and other fields. Assay kits have also played a very significant role in this research field.

• Cell Signaling Assays

  Cell signal is a relevant carrier and a form of mutual information transmission between cells. Most of the communication between cells is transmitted through signal molecules. It is a reaction of antigens (signal molecules) and receptors on or in the cell membrane. Cell signaling assays are essential for many types of research. The assay kit can quickly carry out the cell signaling research.

  The features and advantages of the assay kits are as follows:

  1. High sensitivity and efficiency.
  2. Low cost and simple operation.
  3. Diverse samples, such as plasma, urine, cell supernatant, etc.
  4. The test can be used for many different animal species.
  5. The standard range is wide, allowing the use of high-concentration samples.

Our products are applicable in many research fields and species. All the assay kits have been strictly verified with easy operation and stable performance, which provide you with reliable experimental results. For any questions, please feel free to contact us.

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Cell Signaling Assays  
Enzyme Activity Assays

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