Product Overview :
Internucleosomal DNA fragmentation is a hallmark of apoptosis in mammalian cells. Apo-BrdU-IHC; Kit is a two-color TUNEL (Terminal deoxynucleotide transferase dUTP Nick End Labeling) assay for labeling DNA breaks to detect apoptotic cells by immunohistochemistry. The kit contains positive/negative control slides for assessing reagent performance; reaction and blocking buffers for processing individual steps in the assay; proteinase K; terminal deoxynucleotidyl tranferase enzyme (TdT), bromodeoxyuridine triphosphate (Br-dUTP), biotin labeled antiBrdU antibody for labeling DNA breaks, horseradish peroxidase streptavidin conjugate, DAB, H2O2/Urea tablets for color generation and methyl green solution for counter staining the cells.
Applications :
The TUNEL-based immunohistochemical staining kit provides complete reagents including positive and negative control slides for convenient detection of DNA fragmentation.
Kit Components :
Control Slides-pos/neg; Blocking Buffer; H2O2/Urea Tablets; Protease K; DAB Tablets; TdT Enzymes; Br-dUTP; 200X Conjugate; 5X Reaction Buffer; Anti-BrdU-Biotin Antibody; Methyl Green
Target Species :
Mammalian
Features & Benefits :
Simple procedure; takes ~ 3 hours;
Fast and convenient;
The assay is sensitive and stable;
The TUNEL-based assay kit provides complete components including positive and negative control cells for convenient detection of DNA fragmentation in cultured cells and tissue sections.
PDF