| Cat# | Product Name | Price |
|---|---|---|
| Kit-0099 | Antioxidant Assay Kit | Inquiry |
| Kit-0100 | Total Antioxidant Capacity Assay Kit | Inquiry |
| Kit-2298 | Total Antioxidant Capacity (TAC) Colorimetric Assay Kit I | Inquiry |
| Kit-2299 | Total Antioxidant Capacity (TAC) Colorimetric Assay Kit II | Inquiry |
| kit-2174 | Total Antioxidant Status Assay Kit | Inquiry |
Antioxidants play a pivotal role in maintaining the body’s overall health and balance. They are compounds that inhibit oxidation, a chemical reaction that can produce harmful molecules, known as free radicals. Antioxidants protect cells from damage due to reactive oxygen species (ROS). The human body naturally produces antioxidants, but these substances also live in foods we consume daily, such as fruits, vegetables, nuts, and even dark chocolate.
The biological activity of an antioxidant refers to how it works in preventing or slowing down the damage to cells caused by free radicals. Free radicals are unstable atoms that can harm cells, causing illness and aging, known as oxidative stress. Antioxidants perform their roles by neutralizing these harmful free radicals. They donate an electron to the free radical, converting it into a stable molecule, a factor that stops the negative chain reaction that free radicals can often start.
Antioxidants also have beneficial roles in modulating signaling pathways, gene expression, and other crucial biological activities. Additionally, antioxidants have anti-inflammatory, anti-carcinogenic, and anti-aging effects, which contribute to the amelioration and prevention of many chronic diseases or conditions like cardiovascular disease, neurodegenerative diseases, and cancer.
For scientists to determine the antioxidant activity in substances, several detection methods are available. One of the most common methods is the 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay. This method detects the reducing potential of an antioxidant by using a stable radical, DPPH, which becomes reduced to a non-radical form in the presence of an antioxidant. The reaction can be monitored by spectrophotometry.
Another widely used method is the Ferric Reducing Antioxidant Power (FRAP) assay. FRAP quantifies the total antioxidant power in biological fluids by measuring the reduction in a ferric tripyridyltriazine complex to its ferrous form at low pH, increased absorbance which can be followed spectrophotometrically.
Lastly, the 2,2'-Azino-bis (3-Ethylbenzothiazoline-6-Sulfonic Acid) or ABTS method is a rapid and reliable assay that measures the ability of antioxidants to scavenge the ABTS radical cation. This method has high sensitivity and can be applied to both hydrophilic and lipophilic antioxidants.
In addition to the methods mentioned above, several other techniques are employed in detecting antioxidant function such as the Trolox equivalent antioxidant capacity (TEAC) assay, which measures the antioxidant capacity in both hydrophilic and hydrophobic environments.
In the Oxygen Radical Absorbance Capacity (ORAC) assay, the antioxidant capacity is measured based on the antioxidant's ability to scavenge peroxyl radicals generated from thermal decomposition of AAPH. This capacity is then compared to Trolox, a water-soluble vitamin E analog, hence terminologies like ‘Trolox equivalents’.
To facilitate the detection of antioxidant activity and function, multiple companies offer activity/detection kits. Some of these include:
These kits provide measurable, reliable, and often times highly sensitive assays to determine the antioxidant capacity in a wide variety of samples, aiding further research in the field of antioxidants and their beneficial properties.
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