ShortName :
Amylase Assay Kit
Product Overview :
Amylase Assay Kit detects activity of α-amylase through a two-step reaction.
In the amylase assay protocol, alpha-Amylase will cleave the substrate ethylidene-pNPG7
to produce smaller fragments that are eventually modified by alpha-glucosidase.
This causes the release of a chromophore that can then be measured at OD = 405 nm.
The assay can detect α-amylase content as low as 0.2 mU.
Storage :
Store kit at -20ºC in the dark immediately upon receipt. Kit has a storage time of 1 year
from receipt, providing components have not been reconstituted.
Refer to list of materials supplied for storage conditions of individual components.
Observe the storage conditions for individual prepared components. Aliquot
components in working volumes before storing at the recommended temperature.
Reconstituted components are stable for 2 months.
Kit Components :
Amylase Assay Buffer: 55 mL -20°C
Amylase Substrate Mix: 5 mL -20°C
Amylase Positive Control (Lyophilized): 1 vial -20°C
Nitrophenol Standard (2mM): 150 μL -20°C
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