Product Overview :
The assay is initiated with the enzymatic hydrolysis of the glucoside by α-Galactosidase. The enzyme catalysed reaction products p-nitrophenol, can be measured at a colorimetric readout at 400 nm.
Description :
Alpha-galactosidase is a glycoside hydrolase enzyme that hydrolyses the terminal alpha-galactosyl moieties from glycolipids and glycoproteins. It is encoded by the GLA gene. Two recombinant forms of alpha-galactosidase are called agalsidase alfa (INN) and agalsidase beta (INN). This enzyme is a homodimeric glycoprotein that hydrolyses the terminal alpha-galactosyl moieties from glycolipids and glycoproteins. It predominantly hydrolyzes ceramide trihexoside, and it can catalyze the hydrolysis of melibiose into galactose and glucose.
Storage :
Shipped and store at 4 °C for 6 months.
Synonyms :
Alpha-Galactosidase Kit; Alpha-Galactosidase Assay; Alpha-Galactosidase; EC 3.2.1.22; Melibiase; GLA; GALA; 9025-35-8
Kit Components :
96-Well Microplate: 1 plate
Assay Buffer: 100 ml x 1; 4 °C
Reaction Buffer: 4 ml x 1; 4 °C
Substrate: Powder x 1; -20 °C
Stop Solution: 15 ml x 1; 4 °C
Standard (5μmol/mL p-nitrophenol solution): 1 ml x 1; 4 °C
Note:
Substrate: For each tube, add 2.5 ml distilled water to dissolve before use.
Target Species :
Detection and Quantification of Alpha-Galactosidase (α-GAL) Activity in Tissue extracts, Cell lysate, Cell culture media and Other biological fluids Samples.
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